Haspin histone
WebOct 8, 2010 · Here, we show that the phosphorylation of histone H3–threonine 3 (H3-pT3) mediated by Haspin cooperates with Bub1-mediated histone 2A–serine 121 (H2A-S121) phosphorylation in targeting the CPC to the inner centromere in fission yeast and human cells. H3-pT3 promotes nucleosome binding of survivin, whereas phosphorylated H2A …
Haspin histone
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WebHaspin, a nuclear and chromosome-associated serine/threonine (S/T) kinase, is responsible for mitotic phosphorylation of Thr-3 of histone H3. Haspin bears recognizable similarity to the eukaryotic protein kinase (ePK) fold, but its sequence is highly divergent and there is therefore considerable interest in its structural organization. WebDec 1, 2009 · We bacterially expressed and purified several fragments of the catalytic domain of Haspin. A proteolytically resistant fragment, encompassing residues 452–798 …
WebNov 29, 2024 · Haspin is an atypical serine/threonine protein kinase essential to mitosis. Unlike other protein kinases, its kinase domain does not require phosphorylation in order to be activated and bears very high substrate specificity and selectivity. Few substrates have been identified so far. Haspin phosphorylation on threonine 3 of Histone H3 from … WebNov 4, 2010 · Histone H3 (see 602810) is phosphorylated on thr3 during prophase of mitotic cells, and it is dephosphorylated during anaphase. Dai et al. (2005) found that haspin phosphorylated H3 thr3 in human cell lines in vitro. Depletion of haspin by RNA interference revealed that haspin was required for H3 thr3 phosphorylation in mitotic cells.
WebFeb 2, 2005 · Haspin is a histone H3 kinase. We next examined the in vitro kinase activity of haspin. For these experiments we prepared a control haspin protein (myc-haspin … WebMar 21, 2024 · Entrez Gene Summary for HASPIN Gene. Enables ATP binding activity and histone kinase activity (H3-T3 specific). Involved in histone H3-T3 phosphorylation …
WebDec 8, 2009 · Haspin phosphorylates histone H3 during mitosis, raising the question of how it is regulated during the cell cycle. Unlike mitotic kinases such as Aurora B and Plk1 that are degraded at the end of mitosis (Nigg 2001 ), human haspin is expressed at near-constant levels throughout the cell cycle (Dai et al. 2005 ; Wang F and Higgins JMG ...
WebIntroduction. Ovarian cancer is one of the most common malignant tumors in gynecology. The incidence of ovarian cancer ranks third, while the mortality rate is the highest among female malignant tumors of the reproductive system. 1 Ovarian cancer has the characteristics of concealed onset, high malignancy, and easy metastasis, which causes … buss and companyWebAug 1, 2011 · Very recently, the mechanism of Haspin action has been revealed in three studies showing that histone H3T3 phosphorylation is … bus sandwich to dealWebApr 14, 2024 · HASPIN is a serine/threonine kinase that regulates mitosis by phosphorylating histone H3 at threonine 3. The expression levels of HASPIN in various … bus sandyhills to rockcliffeWebNov 12, 2024 · Inhibition of Haspin leads to a decrease in Histone H3 phosphorylation and prevents Histone H3 binding to survivin, thus providing mechanistic insight of how Sangivamycin targets cell ... bus sandyhills to kippfordWebMay 1, 2024 · Under a structural point of view, Haspin kinase domain shows poor sequence homology with eukaryotic protein kinases (ePKs), bearing several peculiar inserts [7, 8] including an atypical activation segment that specifically recognizes the basic histone tail. Moreover, Haspin lacks typical elements of ePKs such as the Asp-Phe-Gly ATP/Mg2+ … bus sandwich to ramsgateWebFeb 15, 2014 · To date, histone H3 is the only confirmed Haspin substrate. We used a combination of biochemical, pharmacological, and mass spectrometric approaches to study the consequences of Haspin inhibition in mitotic cells. We quantified 3964 phosphorylation sites on chromatin-associated proteins and identified a Haspin protein-protein interaction … bussan financeWebFeb 1, 2024 · Haspin overexpression or deletion results in defective mitosis. It has been shown that Haspin inhibitors have potent anti-tumoral effects. Given that the only Haspin substrate is threonine 3 of histone 3, inhibition of Haspin might have fewer adverse effects compared with other anticancer agents. bus sandwich to dover