Ea.hy926 thrombomodulin elisa
WebEA.hy926 is a somatic hybrid cell that can be used for cardiovascular disease research. This cell line was deposited by CS Edgell. Product category Human cells Organism Homo sapiens, human Cell type somatic cell hybrid Morphology endothelial Applications 3D cell culture Cardiovascular disease research Product format Frozen Storage conditions WebOct 5, 2024 · A modified ELISA 33 was used to detect the binding of biotinylated HS ... EA.hy926 cells were grown to 80% confluency in a six-well plate with 2 ml of complete DMEM and then treated with 0 ...
Ea.hy926 thrombomodulin elisa
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WebJan 9, 2007 · EA.hy926 were incubated for 1 h with buffer (control) or L. intermedia venom (venom) and cell supernatants were analyzed by Western blotting under non-reducing conditions, for the presence of TM (A) and EPCR (B). A detergent lysate of untreated EA.hy926 cells (lysate) was used as an indicator for the native molecular mass of TM … WebRecombinant human soluble thrombomodulin (rTM) counteracted capillary leakage and alleviated edema in individuals with sinusoidal obstruction syndrome and engraftment …
WebThrombomodulin (TM) is a glycosylated type I transmembrane ... EA.hy926 cells were purchased from ATCC (Manassas, VA, USA) and ... with a murine TM ELISA kit (Uscn Life Science, Tokyo, Japan) and ... WebDec 9, 2024 · Abstract Endoglin, a co-receptor of TGF-β family growth factors, is an endothelial cells marker. In our previous work we have demonstrated that monoclonal …
WebTherefore, adhesion of monocytoid THP-1 cells to a vascular endothelial-like cell line (EA.hy926 cells) was quantified. Under quiescent conditions or after exposure of the EA.hy926 cells to TNF- α , the human recombinant lectin-like domain of TM significantly interfered with monocyte adhesion in a dose-dependent manner ( Fig. 6 ). WebHuman Thrombomodulin ELISA Kit (ab214029) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Thrombomodulin protein in cell culture extracts, cell culture …
WebMar 22, 2016 · Presence of soluble forms of VEGF-A in UC-MSC-conditioned media was measured by ELISA. Effects of the conditioned media on human umbilical vein-derived endothelial EA.hy926 cells proliferation were measured by MTT-assay; changes in cell motility and directed migration were assessed by scratch wound healing and transwell …
WebJun 9, 2024 · GEO help: Mouse over screen elements for information. The expression profile in EA.hy926 cells after exposure to hypoxic conditions was evaluated by RNA-sequencing. Hypoxia is an imbalance between oxygen supply and demand that occurs in embryonic development, as well as in pathological situations such as cancer, ischemic heart … how many gallons of water per goldfishWebDec 18, 2024 · We monitored cGAS/STING signaling upon ISD90 or 2′,3′-cGAMP stimulation in EA.hy926 cells by western blotting, RT-PCR, and ELISA analyses to … how many gallons of water per weekWebThrombomodulin (TM), also known as CD141 and BDCA3, is a transmembrane expressed on vascular endothelial cells (EC), arterial smooth muscle cells, monocytes, and macrophages. It binds Thrombin and enhances the Thrombin-mediated activation of the anti-coagulant Protein C and the anti-fibrinolytic TAFI/Carboxypeptidase B2. how many gallons of water should we drinkWebconfluent EA.hy926 monolayers. Briefly, EA.hy926 cells in 24-well plates were preincubated with 0.2 mL of Opti-MEM medium containing 0.1% BSA, 0.1 lM protein C … how many gallons of water to flush a commodeWebIntercellular adhesion molecule (ICAM) and von Willebrand factor (vWF) were measured in cell supernatants by ELISA. Aptamer HBA7 was added 30–60 min after CTH. n = 4–7. (E) HBA7 prevents histone-mediated platelet activation. ... EA.hy926 cells were pretreated with different 4-oxo-flavonoids at a series of concentration (5, 10, 20, 40 and 80 ... how many gallons of water to fill a hot tubWebMTT assays already revealed some CA-4 characteristics such as selectivity for EA.Hy926 endothelial hybrid cells and a reduced efficacy against HT-29 colon carcinoma cells [33]. … how many gallons of water to flush toiletWebMar 22, 2014 · Background A discontinuous dose response relationship is a major characteristic of the anti-inflammatory effects of low-dose X-irradiation therapy. Although recent data indicate an involvement of a variety of molecular mechanisms in these characteristics, the impact of reactive oxygen species (ROS) production to give rise or … how many gallons of water to drink